Hotspot

rpl32-trnL-UAG

SSC · 113759-114684 · 926 bp

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Pi 0.0347
MarkerSeek score 70.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
926
variable_sites
59
pi
0.0347
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.92
markerseek_score
70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 ATTTACCGGATCCTACCCCC AGCGTGTCTACCGATTTCAC 55.0 50.0 58.3 58.0 1613-2340 bp 1.0 85.7
rpl32-trnL-UAG_p2 ATTTACCGGATCCTACCCCC CAGCGTGTCTACCGATTTCA 55.0 50.0 58.3 58.0 1614-2341 bp 1.0 85.7
rpl32-trnL-UAG_p3 AGGGATATTGGGCAGCCTTA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.2 58.0 984-1061 bp 0.833333 80.9
rpl32-trnL-UAG_p4 AGGGATATTGGGCAGCCTTA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.2 58.0 985-1062 bp 0.833333 80.9
rpl32-trnL-UAG_p5 AGGGATATTGGGCAGCCTTA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.2 57.9 994-1071 bp 0.833333 79.9