Hotspot

ndhF-rpl32

SSC · 113066-113593 · 528 bp

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Pi 0.0325
MarkerSeek score 71.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
528
variable_sites
35
pi
0.0325
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.97
markerseek_score
71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GTTGCCGACGGAAAAAGAAG AGGCTTTTGTCGATTGGGTT 50.0 45.0 58.0 58.0 1323-2047 bp 1.0 87.5
ndhF-rpl32_p2 TGCCGACGGAAAAAGAAGAA AGGCTTTTGTCGATTGGGTT 45.0 45.0 58.0 58.0 1321-2045 bp 1.0 87.4
ndhF-rpl32_p3 TTGCCGACGGAAAAAGAAGA AGGCTTTTGTCGATTGGGTT 45.0 45.0 58.0 58.0 1322-2046 bp 1.0 87.4
ndhF-rpl32_p4 CCGTGTGAACCAAAACCCTA AGGCTTTTGTCGATTGGGTT 50.0 45.0 58.0 58.0 1729-2453 bp 1.0 86.4
ndhF-rpl32_p5 CCGTGTGAACCAAAACCCTA GGCTTTTGTCGATTGGGTTG 50.0 50.0 58.0 57.9 1728-2452 bp 1.0 86.0