Hotspot

accD-psaI

LSC · 59409-59889 · 481 bp

Back to Sium

Pi 0.0237
MarkerSeek score 54.70
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
481
variable_sites
24
pi
0.0237
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.94
markerseek_score
54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 AAGCCGTTCAAACAGGCATA TTTTCGCGACCCCGTTATAG 45.0 50.0 58.1 58.1 1438-1673 bp 1.0 85.0
accD-psaI_p2 GATCCGGATACTTGGGATGC TTTTCGCGACCCCGTTATAG 55.0 50.0 57.9 58.1 1562-1797 bp 1.0 84.9
accD-psaI_p3 AAGCCGTTCAAACAGGCATA TGGGGCCCATCAGATCTAAA 45.0 50.0 58.1 58.1 1129-1164 bp 0.666667 70.0
accD-psaI_p4 AAGCCGTTCAAACAGGCATA TTTACCCCCATTACCCCCAT 45.0 50.0 58.1 58.0 1304 bp 0.166667 45.8
accD-psaI_p5 GATCCGGATACTTGGGATGC TTTACCCCCATTACCCCCAT 55.0 50.0 57.9 58.0 1428 bp 0.166667 45.7