Hotspot

trnE-UUC-trnT-GGU

LSC · 31178-32060 · 883 bp

Back to Sium

Pi 0.044
MarkerSeek score 72.10
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
883
variable_sites
69
pi
0.044
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.82
markerseek_score
72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 ACCCAAGAAGAGCCCATTTG GAACCGATGACTTACGCCTT 50.0 50.0 58.1 58.0 638-1130 bp 1.0 89.7
trnE-UUC-trnT-GGU_p2 ACCCAAGAAGAGCCCATTTG TGGTGATTCCAGAGACGACT 50.0 50.0 58.1 58.1 892-1388 bp 1.0 88.6
trnE-UUC-trnT-GGU_p3 AAACCGGAGTCAGAAGGAGA GAACCGATGACTTACGCCTT 50.0 50.0 58.0 58.0 1260-1578 bp 1.0 88.1
trnE-UUC-trnT-GGU_p4 AAACCGGAGTCAGAAGGAGA TGGTGATTCCAGAGACGACT 50.0 50.0 58.0 58.1 1505-1836 bp 1.0 87.5
trnE-UUC-trnT-GGU_p5 AAACCGGAGTCAGAAGGAGA CCGATGACTTACGCCTTACC 50.0 55.0 58.0 58.2 1257-1575 bp 1.0 87.0