Hotspot

atpI-rps2

LSC · 15317-15600 · 284 bp

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Pi 0.0286
MarkerSeek score 62.40
Primer available yes
Label atpI-rps2

Feature Metadata

label_name
atpI-rps2
feature_type
igs
region
LSC
length_bp
284
variable_sites
17
pi
0.0286
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI-rps2_p1 AACCCGTTTAGTGCGTTGAT TGTGAAGGGCGTTCTAGCTA 45.0 50.0 58.1 58.4 361-491 bp 1.0 82.6
atpI-rps2_p2 AACCCGTTTAGTGCGTTGAT TTTGTGAAGGGCGTTCTAGC 45.0 50.0 58.1 58.5 363-493 bp 1.0 82.3
atpI-rps2_p3 AACCCGTTTAGTGCGTTGAT GCAATTTGTGAAGGGCGTTC 45.0 50.0 58.1 58.6 367-497 bp 1.0 81.4
atpI-rps2_p4 ATGTTGGCCTACTTCCACAC TGTGAAGGGCGTTCTAGCTA 50.0 50.0 57.8 58.4 395-525 bp 1.0 81.3
atpI-rps2_p5 ATGTTGGCCTACTTCCACAC TTTGTGAAGGGCGTTCTAGC 50.0 50.0 57.8 58.5 397-527 bp 1.0 81.0