Hotspot

psbK-psbI

LSC · 7858-8254 · 397 bp

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Pi 0.0148
MarkerSeek score 60.20
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
397
variable_sites
15
pi
0.0148
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCTGCTGTAAG ACGCCCCGGATCATTAGATA 50.0 50.0 57.9 58.1 517-518 bp 1.0 81.5
psbK-psbI_p2 GTTTGGCAAGCTGCTGTAAG TACGCCCCGGATCATTAGAT 50.0 50.0 57.9 58.1 518-519 bp 1.0 81.5
psbK-psbI_p3 GTTTGGCAAGCTGCTGTAAG TTACGCCCCGGATCATTAGA 50.0 50.0 57.9 58.3 519-520 bp 1.0 79.7
psbK-psbI_p4 TGGCAAGCTGCTGTAAGTTT ACGCCCCGGATCATTAGATA 45.0 50.0 58.3 58.1 514-515 bp 1.0 79.5
psbK-psbI_p5 TGGCAAGCTGCTGTAAGTTT TACGCCCCGGATCATTAGAT 45.0 50.0 58.3 58.1 515-516 bp 1.0 79.5