Hotspot

rpl16

LSC · 78205-79584 · 1380 bp

Back to Sisyrinchium

Pi 0.0165

MarkerSeek score 57.40

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1380
variable_sites: 22
pi: 0.0165
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TTCATCCTTAATACCCCTCT	TCTGTCTAGTACCAATAATCGAACA	40.0	36.0	52.3	57.4	1482-1490 bp	1.0	42.2
rpl16_p2	TTTCATCCTTAATACCCCTCT	TCTGTCTAGTACCAATAATCGAACA	38.1	36.0	53.3	57.4	1483-1491 bp	1.0	42.2
rpl16_p3	TTTTCATCCTTAATACCCCTCT	TCTGTCTAGTACCAATAATCGAACA	36.4	36.0	54.2	57.4	1484-1492 bp	1.0	42.2
rpl16_p4	TTCATCCTTAATACCCCTCT	TCGAACAAACAAAAATGAACA	40.0	28.6	52.3	53.1	1464-1472 bp	1.0	42.2
rpl16_p5	TTCATCCTTAATACCCCTCT	CTGTCTAGTACCAATAATCGAACA	40.0	37.5	52.3	56.1	1481-1489 bp	1.0	42.2