Hotspot

rbcL

LSC · 53082-54542 · 1461 bp

Back to Sisyrinchium

Pi 0.00684

MarkerSeek score 52.60

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1461
variable_sites: 10
pi: 0.00684
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TCAAAGGTTTCATTCACGCC	TGGGCCGAGATTAATTGCAA	45.0	45.0	56.9	57.9	1587 bp	1.0	70.8
rbcL_p2	TCAAAGGTTTCATTCACGCC	TTGGGCCGAGATTAATTGCA	45.0	45.0	56.9	57.9	1588 bp	1.0	70.8
rbcL_p3	CAAAGGTTTCATTCACGCCT	TGGGCCGAGATTAATTGCAA	45.0	45.0	56.9	57.9	1586 bp	1.0	70.7
rbcL_p4	CAAAGGTTTCATTCACGCCT	TTGGGCCGAGATTAATTGCA	45.0	45.0	56.9	57.9	1587 bp	1.0	70.7
rbcL_p5	GTCAAAGGTTTCATTCACGCC	TGGGCCGAGATTAATTGCAA	47.6	45.0	58.3	57.9	1588 bp	1.0	68.7