Hotspot

trnI-CAU-rpl23

IRa · 148766-148930 · 165 bp

Back to Sisyrinchium

Pi 0.0125

MarkerSeek score 62.60

Primer available yes

Label trnI-CAU-rpl23

Feature Metadata

label_name: trnI-CAU-rpl23
feature_type: igs
region: IRa
length_bp: 165
variable_sites: 2
pi: 0.0125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-rpl23_p1	GAATGGTTAAAGCGCCCAAC	TGATTGTTCCCCAATAACCGA	50.0	42.9	58.0	57.3	284-289 bp	1.0	68.1
trnI-CAU-rpl23_p2	CGATGAGCCCTGTTAAGCAT	TGATTGTTCCCCAATAACCGA	50.0	42.9	58.0	57.3	312-317 bp	1.0	67.7
trnI-CAU-rpl23_p3	AGCCCTGTTAAGCATCCATG	TGATTGTTCCCCAATAACCGA	50.0	42.9	57.9	57.3	307-312 bp	1.0	67.5
trnI-CAU-rpl23_p4	CCTGTTAAGCATCCATGGCT	TGATTGTTCCCCAATAACCGA	50.0	42.9	57.9	57.3	304-309 bp	1.0	67.5
trnI-CAU-rpl23_p5	TTCACGATGTACGATGAGCC	TGATTGTTCCCCAATAACCGA	50.0	42.9	57.8	57.3	323-328 bp	1.0	66.3