Hotspot

ndhF-rpl32

SSC · 109185-110130 · 946 bp

Back to Sisyrinchium

Pi 0.00742

MarkerSeek score 63.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 946
variable_sites: 7
pi: 0.00742
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GAAGTCCGAGTCCCATTGAC	TGTGGAAACCGATTTAGCTA	55.0	40.0	58.0	54.4	1145-1214 bp	1.0	51.6
ndhF-rpl32_p2	CAGAAGTCCGAGTCCCATTG	TGTGGAAACCGATTTAGCTA	55.0	40.0	58.0	54.4	1147-1216 bp	1.0	51.6
ndhF-rpl32_p3	AGAAGTCCGAGTCCCATTGA	TGTGGAAACCGATTTAGCTA	50.0	40.0	58.1	54.4	1146-1215 bp	1.0	51.2
ndhF-rpl32_p4	GAAGTCCGAGTCCCATTGAC	TGCAGCAAAATATCCCTTTTTCT	55.0	34.8	58.0	57.3	1109-1178 bp	1.0	50.6
ndhF-rpl32_p5	CAGAAGTCCGAGTCCCATTG	TGCAGCAAAATATCCCTTTTTCT	55.0	34.8	58.0	57.3	1111-1180 bp	1.0	50.6