Hotspot

petD-rpoA

LSC · 74753-74930 · 178 bp

Back to Sisyrinchium

Pi 0.0412
MarkerSeek score 78.20
Primer available yes
Label petD-rpoA

Feature Metadata

label_name
petD-rpoA
feature_type
igs
region
LSC
length_bp
178
variable_sites
7
pi
0.0412
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD-rpoA_p1 TGGAGCAACATTACCTATTGA TGGACACTCTACAGAAACGT 38.1 45.0 54.6 56.1 292-293 bp 1.0 43.3
petD-rpoA_p2 TTGGAGCAACATTACCTATTGA TGGACACTCTACAGAAACGT 36.4 45.0 55.4 56.1 293-294 bp 1.0 43.3
petD-rpoA_p3 TGGAGCAACATTACCTATTGA TGGACACTCTACAGAAACGTT 38.1 42.9 54.6 56.9 292-293 bp 1.0 43.3
petD-rpoA_p4 TGGAGCAACATTACCTATTGA TTGGACACTCTACAGAAACGT 38.1 42.9 54.6 56.9 293-294 bp 1.0 43.3
petD-rpoA_p5 TGGAGCAACATTACCTATTGAT TGGACACTCTACAGAAACGT 36.4 45.0 55.2 56.1 292-293 bp 1.0 43.3