Hotspot

psbT-pbf1

LSC · 71273-71340 · 68 bp

Back to Sisyrinchium

Pi 0.0294

MarkerSeek score 66.50

Primer available yes

Label psbT-pbf1

Feature Metadata

label_name: psbT-pbf1
feature_type: igs
region: LSC
length_bp: 68
variable_sites: 2
pi: 0.0294
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-pbf1_p1	TTTTTCGAGAACCGCCGAA	ATCCATTCGAAGAACACGGG	47.4	50.0	58.0	58.0	132-135 bp	1.0	72.9
psbT-pbf1_p2	TTTTTCGAGAACCGCCGAAG	ATCCATTCGAAGAACACGGG	50.0	50.0	59.1	58.0	132-135 bp	1.0	71.9
psbT-pbf1_p3	TTTTTCGAGAACCGCCGAA	ACCCTAGTCGCCATCTTCAT	47.4	50.0	58.0	58.2	226-229 bp	1.0	71.0
psbT-pbf1_p4	TTTTTCGAGAACCGCCGAA	GGGTACGCCTTATATACCGC	47.4	55.0	58.0	57.7	181-184 bp	1.0	70.5
psbT-pbf1_p5	TTTTTCGAGAACCGCCGAAG	ACCCTAGTCGCCATCTTCAT	50.0	50.0	59.1	58.2	226-229 bp	1.0	70.0