Hotspot

petA-psbJ

LSC · 60580-61486 · 907 bp

Back to Sisyrinchium

Pi 0.0146

MarkerSeek score 68.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 907
variable_sites: 13
pi: 0.0146
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.0	58.3	1059-1064 bp	1.0	79.4
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	CATATTCCGGGTTGGGTTCA	50.0	50.0	58.0	57.6	1055-1060 bp	1.0	78.5
petA-psbJ_p3	ATCCATTACGTGTCCAAGGC	GTTCATATTCCGGGTTGGGT	50.0	50.0	58.0	57.6	1058-1063 bp	1.0	78.4
petA-psbJ_p4	CATTACGTGTCCAAGGCCTT	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.2	58.3	1056-1061 bp	1.0	78.3
petA-psbJ_p5	CATTACGTGTCCAAGGCCTT	CATATTCCGGGTTGGGTTCA	50.0	50.0	58.2	57.6	1052-1057 bp	1.0	77.3