Hotspot

ycf4-cemA

LSC · 57942-58710 · 769 bp

Back to Sisyrinchium

Pi 0.00781

MarkerSeek score 62.70

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 769
variable_sites: 6
pi: 0.00781
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CAAAAGGCTGCCGAATTAGC	AGGATAAAGAAACCCACCAGG	50.0	47.6	58.1	57.3	902-904 bp	1.0	66.0
ycf4-cemA_p2	AATTGAACAAAAGGCTGCCG	AGGATAAAGAAACCCACCAGG	45.0	47.6	57.8	57.3	909-911 bp	1.0	65.5
ycf4-cemA_p3	CAAAAGGCTGCCGAATTAGC	CCACCAATTAGTAACCCAAGGT	50.0	45.5	58.1	58.0	940-942 bp	1.0	63.6
ycf4-cemA_p4	CAAAAGGCTGCCGAATTAGC	AAGGATAAAGAAACCCACCAGG	50.0	45.5	58.1	58.0	903-905 bp	1.0	63.5
ycf4-cemA_p5	GAACAAAAGGCTGCCGAATT	AGGATAAAGAAACCCACCAGG	45.0	47.6	57.6	57.3	905-907 bp	1.0	63.2