Hotspot

accD-psaI

LSC · 56586-56928 · 343 bp

Back to Sisyrinchium

Pi 0.0289

MarkerSeek score 73.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 343
variable_sites: 9
pi: 0.0289
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GGTTCACAAGTGGCTGAGTA	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.5	58.0	511-533 bp	1.0	80.8
accD-psaI_p2	GGTTCACAAGTGGCTGAGTA	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.5	57.9	517-539 bp	1.0	80.2
accD-psaI_p3	GGTTCACAAGTGGCTGAGTAT	CCATTGCAATTGCCGGAAAT	47.6	45.0	57.9	58.0	511-533 bp	1.0	76.7
accD-psaI_p4	GGTTCACAAGTGGCTGAGTAT	AAGAAGCCATTGCAATTGCC	47.6	45.0	57.9	57.9	517-539 bp	1.0	76.1
accD-psaI_p5	GGTTCACAAGTGGCTGAGTA	CCGGAAATACTAGGCCCACT	50.0	55.0	57.5	58.9	499-521 bp	1.0	74.1