Hotspot

rpl32-trnL-CUA

SSC · 113017-113655 · 639 bp

Back to Sisymbrium

Pi 0.0307
MarkerSeek score 69.80
Primer available yes
Label rpl32-trnL-CUA

Feature Metadata

label_name
rpl32-trnL-CUA
feature_type
igs
region
SSC
length_bp
639
variable_sites
48
pi
0.0307
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-CUA_p1 TCCACAGGTAATTCAAAAAGT AGCAGCGTGTCTACCAATTT 33.3 45.0 53.2 57.8 678-766 bp 1.0 49.2
rpl32-trnL-CUA_p2 TCCACAGGTAATTCAAAAAGT CTAAGAGCAGCGTGTCTACC 33.3 55.0 53.2 57.8 683-771 bp 1.0 49.2
rpl32-trnL-CUA_p3 TCCACAGGTAATTCAAAAAGT TCCTAAGAGCAGCGTGTCTA 33.3 50.0 53.2 57.9 685-773 bp 1.0 49.1
rpl32-trnL-CUA_p4 TCCACAGGTAATTCAAAAAGT CCTAAGAGCAGCGTGTCTAC 33.3 55.0 53.2 57.8 684-772 bp 1.0 49.1
rpl32-trnL-CUA_p5 TCCACAGGTAATTCAAAAAGTT TCCTAAGAGCAGCGTGTCTA 31.8 50.0 54.1 57.9 685-773 bp 1.0 49.1