Hotspot

trnR-CGU-trnN-AAC

IRb · 107759-108375 · 617 bp

Back to Sisymbrium

Pi 0.00866

MarkerSeek score 62.40

Primer available yes

Label trnR-CGU-trnN-AAC

Feature Metadata

label_name: trnR-CGU-trnN-AAC
feature_type: igs
region: IRb
length_bp: 617
variable_sites: 14
pi: 0.00866
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-CGU-trnN-AAC_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	745-773 bp	1.0	81.5
trnR-CGU-trnN-AAC_p2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.7	58.0	782-810 bp	1.0	80.8
trnR-CGU-trnN-AAC_p3	CAGAGGATTAGAGCACGTGG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.8	58.1	786-814 bp	1.0	80.6
trnR-CGU-trnN-AAC_p4	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	696-724 bp	1.0	80.5
trnR-CGU-trnN-AAC_p5	GAACAGATCCAGTGGAGACG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.7	58.1	823-851 bp	1.0	79.9