Hotspot

atpB-rbcL

LSC · 53570-54354 · 785 bp

Back to Sisymbrium

Pi 0.0216

MarkerSeek score 58.60

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 785
variable_sites: 44
pi: 0.0216
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ACCTCTGGATCCGAAGTAGT	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.2	58.0	865-872 bp	1.0	69.9
atpB-rbcL_p2	ACCTCTGGATCCGAAGTAGT	ACCAGCTTTGAATCCAACACT	50.0	42.9	57.2	58.1	864-871 bp	1.0	69.7
atpB-rbcL_p3	TGAAACCTCTGGATCCGAAG	CACCAGCTTTGAATCCAACAC	50.0	47.6	56.9	58.0	869-876 bp	1.0	68.1
atpB-rbcL_p4	GAAACCTCTGGATCCGAAGT	CACCAGCTTTGAATCCAACAC	50.0	47.6	56.9	58.0	868-875 bp	1.0	68.1
atpB-rbcL_p5	TGAAACCTCTGGATCCGAAG	ACCAGCTTTGAATCCAACACT	50.0	42.9	56.9	58.1	868-875 bp	1.0	67.8