Hotspot

trnF-UUC-ndhJ

LSC · 47463-48075 · 613 bp

Back to Sisymbrium

Pi 0.0174

MarkerSeek score 58.60

Primer available yes

Label trnF-UUC-ndhJ

Feature Metadata

label_name: trnF-UUC-ndhJ
feature_type: igs
region: LSC
length_bp: 613
variable_sites: 28
pi: 0.0174
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-UUC-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGCCCCCAATTTTTATGAAA	50.0	35.0	58.1	53.5	650-684 bp	1.0	51.4
trnF-UUC-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TTGCCCCCAATTTTTATGAA	50.0	35.0	58.1	53.5	651-685 bp	1.0	51.4
trnF-UUC-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TTGCCCCCAATTTTTATGAAA	50.0	33.3	58.1	54.5	651-685 bp	1.0	50.6
trnF-UUC-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	TGCCCCCAATTTTTATGAAATACA	50.0	33.3	58.1	57.4	650-684 bp	1.0	50.0
trnF-UUC-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	ATTGCCCCCAATTTTTATGA	50.0	35.0	58.1	53.3	652-686 bp	1.0	49.4