Hotspot

trnT-ACA-trnL-UUA

LSC · 45961-46705 · 745 bp

Back to Sisymbrium

Pi 0.0165

MarkerSeek score 60.00

Primer available yes

Label trnT-ACA-trnL-UUA

Feature Metadata

label_name: trnT-ACA-trnL-UUA
feature_type: igs
region: LSC
length_bp: 745
variable_sites: 30
pi: 0.0165
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-ACA-trnL-UUA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCAATTCC	55.0	55.0	57.9	58.2	795-819 bp	1.0	83.4
trnT-ACA-trnL-UUA_p2	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.9	58.2	798-822 bp	1.0	82.6
trnT-ACA-trnL-UUA_p3	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCAATTCC	50.0	55.0	57.8	58.2	827-851 bp	1.0	82.3
trnT-ACA-trnL-UUA_p4	GGAATCGAACCGATGACCAT	AGTCCGTAGCGTCTACCAAT	50.0	50.0	57.8	58.2	830-854 bp	1.0	81.5
trnT-ACA-trnL-UUA_p5	GCGATGCTCTAACCTCTGAG	TAAGTCCGTAGCGTCTACCA	55.0	50.0	57.9	57.6	800-824 bp	1.0	81.3