Hotspot

ycf3-trnS-UCC

LSC · 43442-44531 · 1090 bp

Back to Sisymbrium

Pi 0.0107

MarkerSeek score 61.00

Primer available yes

Label ycf3-trnS-UCC

Feature Metadata

label_name: ycf3-trnS-UCC
feature_type: igs
region: LSC
length_bp: 1090
variable_sites: 29
pi: 0.0107
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-UCC_p1	CCTTCAGATTGAGCCGACAT	TGTTACGCCTTCAACCACTC	50.0	50.0	57.7	58.1	1125-1160 bp	1.0	79.6
ycf3-trnS-UCC_p2	CCTTCAGATTGAGCCGACAT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.7	58.2	1181-1216 bp	1.0	79.3
ycf3-trnS-UCC_p3	TTCCTTCAGATTGAGCCGAC	TGTTACGCCTTCAACCACTC	50.0	50.0	57.6	58.1	1127-1162 bp	1.0	79.1
ycf3-trnS-UCC_p4	GGGGTCAATTTCTAGTCGCA	TGTTACGCCTTCAACCACTC	50.0	50.0	57.6	58.1	1183-1218 bp	1.0	78.9
ycf3-trnS-UCC_p5	TTCCTTCAGATTGAGCCGAC	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.6	58.2	1183-1218 bp	1.0	78.8