Hotspot

trnC-UGC-petN

LSC · 27345-27950 · 606 bp

Back to Sisymbrium

Pi 0.0164

MarkerSeek score 57.60

Primer available yes

Label trnC-UGC-petN

Feature Metadata

label_name: trnC-UGC-petN
feature_type: igs
region: LSC
length_bp: 606
variable_sites: 26
pi: 0.0164
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-UGC-petN_p1	TTTCCCCAGTTCAAATCCGG	ACTTCTTCCCCACACTACGA	50.0	50.0	58.1	58.0	707-743 bp	1.0	83.2
trnC-UGC-petN_p2	TTTCCCCAGTTCAAATCCGG	CACTTCTTCCCCACACTACG	50.0	55.0	58.1	57.9	708-744 bp	1.0	82.6
trnC-UGC-petN_p3	TTTCCCCAGTTCAAATCCGG	CTTCCCCACACTACGAGAGA	50.0	55.0	58.1	58.2	703-739 bp	1.0	81.9
trnC-UGC-petN_p4	TTTCCCCAGTTCAAATCCGG	TCTTCCCCACACTACGAGAG	50.0	55.0	58.1	58.2	704-740 bp	1.0	81.9
trnC-UGC-petN_p5	TTTCCCCAGTTCAAATCCGG	TTCCCCACACTACGAGAGAG	50.0	55.0	58.1	58.2	702-738 bp	1.0	81.9