Hotspot

trnS-AGC-trnG-GGA

LSC · 7815-8440 · 626 bp

Back to Sisymbrium

Pi 0.0252
MarkerSeek score 68.00
Primer available yes
Label trnS-AGC-trnG-GGA

Feature Metadata

label_name
trnS-AGC-trnG-GGA
feature_type
igs
region
LSC
length_bp
626
variable_sites
39
pi
0.0252
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-AGC-trnG-GGA_p1 CCAACGCTTTAGTCCACTCA AATCAAACCGAGAGACCCTT 50.0 45.0 57.8 56.5 734-769 bp 1.0 73.2
trnS-AGC-trnG-GGA_p2 CCAACGCTTTAGTCCACTCA ATCAAACCGAGAGACCCTTT 50.0 45.0 57.8 56.5 733-768 bp 1.0 73.2
trnS-AGC-trnG-GGA_p3 AACGCTTTAGTCCACTCAGC AATCAAACCGAGAGACCCTT 50.0 45.0 58.2 56.5 732-767 bp 1.0 72.9
trnS-AGC-trnG-GGA_p4 AACGCTTTAGTCCACTCAGC ATCAAACCGAGAGACCCTTT 50.0 45.0 58.2 56.5 731-766 bp 1.0 72.9
trnS-AGC-trnG-GGA_p5 CCAACGCTTTAGTCCACTCA AATCAAACCGAGAGACCCTTT 50.0 42.9 57.8 57.2 734-769 bp 1.0 71.2