Hotspot

trnH-CAC-psbA

LSC · 77-371 · 295 bp

Back to Sisymbrium

Pi 0.0643

MarkerSeek score 71.50

Primer available yes

Label trnH-CAC-psbA

Feature Metadata

label_name: trnH-CAC-psbA
feature_type: igs
region: LSC
length_bp: 295
variable_sites: 43
pi: 0.0643
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-CAC-psbA_p1	CTGCCTTAATCCACTTGGCT	GCTGTTGAGGCTCCATCTAC	50.0	55.0	57.9	58.1	338-353 bp	1.0	90.5
trnH-CAC-psbA_p2	CTGCCTTAATCCACTTGGCT	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	353-368 bp	1.0	90.4
trnH-CAC-psbA_p3	CTGCCTTAATCCACTTGGCT	GGTGTAGCTGGTGTATTCGG	50.0	55.0	57.9	58.1	770-785 bp	1.0	86.7
trnH-CAC-psbA_p4	CTGCCTTAATCCACTTGGCT	GTTGATAGTCAAGGACGCGT	50.0	50.0	57.9	58.0	467 bp	0.166667	45.5
trnH-CAC-psbA_p5	ACTGCCTTAATCCACTTGGC	GTTGATAGTCAAGGACGCGT	50.0	50.0	58.2	58.0	468 bp	0.166667	45.3