Hotspot

ycf1

SSC · 123938-129208 · 5271 bp

Back to Sisymbriopsis

Pi 0.00436

MarkerSeek score 55.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5271
variable_sites: 34
pi: 0.00436
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTCCCCCATACCCCGAATAT	TCGCTAGTTCTAAAGCCAAGT	55.0	42.9	58.3	57.0	267 bp	1.0	63.2
ycf1_p2	TCCCCCATACCCCGAATATT	TCGCTAGTTCTAAAGCCAAGT	50.0	42.9	57.6	57.0	266 bp	1.0	62.3
ycf1_p3	GTCCCCCATACCCCGAATAT	CTCGCTAGTTCTAAAGCCAAGT	55.0	45.5	58.3	58.1	268 bp	1.0	61.6
ycf1_p4	GTCCCCCATACCCCGAATAT	AGCCAAGTTTAACCAAAGAGGA	55.0	40.9	58.3	57.8	254 bp	1.0	61.5
ycf1_p5	TCCCCCATACCCCGAATATT	CTCGCTAGTTCTAAAGCCAAGT	50.0	45.5	57.6	58.1	267 bp	1.0	60.8