Hotspot

ndhH

SSC · 122056-123237 · 1182 bp

Back to Sisymbriopsis

Pi 0.00113

MarkerSeek score 59.50

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 2
pi: 0.00113
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	TCCATATTAGTCCGTAGACCTCT	TCATGCTAGAACGAATCAAGGA	43.5	40.9	57.4	57.3	1297 bp	1.0	40.1
ndhH_p2	GGACAAACATCCATATTAGTCCG	TCATGCTAGAACGAATCAAGGA	43.5	40.9	57.1	57.3	1306 bp	1.0	40.1
ndhH_p3	TCCATATTAGTCCGTAGACCTCT	TCATGCTAGAACGAATCAAGGAA	43.5	39.1	57.4	57.9	1297 bp	1.0	40.1
ndhH_p4	TCCATATTAGTCCGTAGACCTCT	TTCATGCTAGAACGAATCAAGGA	43.5	39.1	57.4	57.9	1298 bp	1.0	40.1
ndhH_p5	ACATCCATATTAGTCCGTAGACC	TCATGCTAGAACGAATCAAGGA	43.5	40.9	57.0	57.3	1300 bp	1.0	40.1