Hotspot

ndhF

SSC · 110537-112777 · 2241 bp

Back to Sisymbriopsis

Pi 0.00178

MarkerSeek score 58.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2241
variable_sites: 6
pi: 0.00178
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GGATGATGAATTCCACGTTCG	TTTCGGTTTGAAATCGCGTG	47.6	45.0	57.4	58.0	2436-2437 bp	1.0	67.3
ndhF_p2	TCCGAGTGAATGGAAAAGACA	TTTCGGTTTGAAATCGCGTG	42.9	45.0	57.2	58.0	2467-2468 bp	1.0	65.9
ndhF_p3	GGATGATGAATTCCACGTTCG	GGTTTGAAATCGCGTGCTTT	47.6	45.0	57.4	58.2	2432-2433 bp	1.0	65.6
ndhF_p4	TCCGAGTGAATGGAAAAGACA	GGTTTGAAATCGCGTGCTTT	42.9	45.0	57.2	58.2	2463-2464 bp	1.0	64.3
ndhF_p5	GATGAATTCCACGTTCGAACA	TTTCGGTTTGAAATCGCGTG	42.9	45.0	56.9	58.0	2432-2433 bp	1.0	63.4