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petA-psbJ

LSC · 62610-63534 · 925 bp

Back to Sisymbriopsis

Pi 0.00362

MarkerSeek score 61.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 925
variable_sites: 5
pi: 0.00362
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGACTTTTGT	GCTGGTATTCTTGTGATCGGT	45.0	47.6	57.6	58.1	1112-1131 bp	1.0	68.8
petA-psbJ_p2	ACGTGTCCAAGGACTTTTGT	AGCTGGTATTCTTGTGATCGG	45.0	47.6	57.6	57.8	1113-1132 bp	1.0	68.1
petA-psbJ_p3	TAGGATCGGTTGTTTTGGCA	GCTGGTATTCTTGTGATCGGT	45.0	47.6	57.4	58.1	1086-1105 bp	1.0	67.6
petA-psbJ_p4	TAGGATCGGTTGTTTTGGCA	AGCTGGTATTCTTGTGATCGG	45.0	47.6	57.4	57.8	1087-1106 bp	1.0	66.8
petA-psbJ_p5	TCCATTACGTGTCCAAGGAC	GCTGGTATTCTTGTGATCGGT	50.0	47.6	57.2	58.1	1118-1137 bp	1.0	66.1