Hotspot

trnT-ACC-psbD

LSC · 31440-32695 · 1256 bp

Back to Sisymbriopsis

Pi 0.00531

MarkerSeek score 62.10

Primer available yes

Label trnT-ACC-psbD

Feature Metadata

label_name: trnT-ACC-psbD
feature_type: igs
region: LSC
length_bp: 1256
variable_sites: 10
pi: 0.00531
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-ACC-psbD_p1	GTGGTAGAGTAACGCCATGG	CAAAAACAAAGCGGTCCCTC	55.0	50.0	58.1	57.9	1405 bp	1.0	79.0
trnT-ACC-psbD_p2	TCAGTGGTAGAGTAACGCCA	CAAAAACAAAGCGGTCCCTC	50.0	50.0	58.1	57.9	1408 bp	1.0	78.8
trnT-ACC-psbD_p3	GGCGTAAGTCATCGGTTCAA	CAAAAACAAAGCGGTCCCTC	50.0	50.0	58.3	57.9	1382 bp	1.0	77.2
trnT-ACC-psbD_p4	TGGCCCTTTTAACTCAGTGG	CAAAAACAAAGCGGTCCCTC	50.0	50.0	57.7	57.9	1421 bp	1.0	77.2
trnT-ACC-psbD_p5	GGCCCTTTTAACTCAGTGGT	CAAAAACAAAGCGGTCCCTC	50.0	50.0	57.7	57.9	1420 bp	1.0	77.2