Hotspot

trnC-UGC-petN

LSC · 27365-28024 · 660 bp

Back to Sisymbriopsis

Pi 0.00708

MarkerSeek score 61.30

Primer available yes

Label trnC-UGC-petN

Feature Metadata

label_name: trnC-UGC-petN
feature_type: igs
region: LSC
length_bp: 660
variable_sites: 7
pi: 0.00708
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-UGC-petN_p1	TTTCCCCAGTTCAAATCCGG	CTTCCCCACACTACGAGAGA	50.0	55.0	58.1	58.2	763-766 bp	1.0	79.1
trnC-UGC-petN_p2	TTTCCCCAGTTCAAATCCGG	TTCCCCACACTACGAGAGAG	50.0	55.0	58.1	58.2	762-765 bp	1.0	79.1
trnC-UGC-petN_p3	GTTTCTATCGTTTTGGCGGC	CTTCCCCACACTACGAGAGA	50.0	55.0	58.1	58.2	819-822 bp	1.0	78.8
trnC-UGC-petN_p4	GTTTCTATCGTTTTGGCGGC	TTCCCCACACTACGAGAGAG	50.0	55.0	58.1	58.2	818-821 bp	1.0	78.8
trnC-UGC-petN_p5	TTTCCCCAGTTCAAATCCGG	CTAGAGTCCACTCCTTCCCC	50.0	60.0	58.1	58.2	776-779 bp	1.0	78.6