Hotspot

trnS-AGC-trnG-GGA

LSC · 7730-8513 · 784 bp

Back to Sisymbriopsis

Pi 0.0127

MarkerSeek score 67.40

Primer available yes

Label trnS-AGC-trnG-GGA

Feature Metadata

label_name: trnS-AGC-trnG-GGA
feature_type: igs
region: LSC
length_bp: 784
variable_sites: 11
pi: 0.0127
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-AGC-trnG-GGA_p1	CCAACGCTTTAGTCCACTCA	TGAATCAAACCGAGAGACCC	50.0	50.0	57.8	57.2	750-894 bp	1.0	77.7
trnS-AGC-trnG-GGA_p2	AACGCTTTAGTCCACTCAGC	TGAATCAAACCGAGAGACCC	50.0	50.0	58.2	57.2	748-892 bp	1.0	77.4
trnS-AGC-trnG-GGA_p3	GCTTTAGTCCACTCAGCCAT	TGAATCAAACCGAGAGACCC	50.0	50.0	57.6	57.2	745-889 bp	1.0	75.7
trnS-AGC-trnG-GGA_p4	TCCAACGCTTTAGTCCACTC	TGAATCAAACCGAGAGACCC	50.0	50.0	57.5	57.2	751-895 bp	1.0	75.4
trnS-AGC-trnG-GGA_p5	CCAACGCTTTAGTCCACTCA	GAATCAAACCGAGAGACCCT	50.0	50.0	57.8	56.9	749-893 bp	1.0	75.3