Hotspot

ccsA

SSC · 118540-119511 · 972 bp

Back to Siraitia

Pi 0.00514

MarkerSeek score 61.60

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 5
pi: 0.00514
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TGAATTGAGGGACCCTTTTT	CATGTTTGTGTTCGTCAGGC	40.0	50.0	54.7	58.0	1042-1048 bp	1.0	54.1
ccsA_p2	TTGAATTGAGGGACCCTTTT	CATGTTTGTGTTCGTCAGGC	40.0	50.0	54.7	58.0	1043-1049 bp	1.0	54.1
ccsA_p3	TGAATTGAGGGACCCTTTTT	CGTCAGGCCCTTTCTTGAAT	40.0	50.0	54.7	58.2	1030-1036 bp	1.0	53.1
ccsA_p4	TTGAATTGAGGGACCCTTTT	CGTCAGGCCCTTTCTTGAAT	40.0	50.0	54.7	58.2	1031-1037 bp	1.0	53.1
ccsA_p5	TTGAATTGAGGGACCCTTTTT	CATGTTTGTGTTCGTCAGGC	38.1	50.0	55.6	58.0	1043-1049 bp	1.0	53.0