Hotspot

ndhH-rps15

SSC · 127323-127425 · 103 bp

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Pi 0.0194

MarkerSeek score 63.70

Primer available yes

Label ndhH-rps15

Feature Metadata

label_name: ndhH-rps15
feature_type: igs
region: SSC
length_bp: 103
variable_sites: 2
pi: 0.0194
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-rps15_p1	ATGAGTCGAAGAACACCGTG	CGGAAAACGCCAACGATTAC	50.0	50.0	57.9	58.1	292 bp	1.0	79.0
ndhH-rps15_p2	ATGAGTCGAAGAACACCGTG	TCGGAAAACGCCAACGATTA	50.0	45.0	57.9	58.2	293 bp	1.0	78.4
ndhH-rps15_p3	CATTGATGGGTGGTGAGGTC	CGGAAAACGCCAACGATTAC	55.0	50.0	58.2	58.1	272 bp	1.0	77.6
ndhH-rps15_p4	CATTGATGGGTGGTGAGGTC	TCGGAAAACGCCAACGATTA	55.0	45.0	58.2	58.2	273 bp	1.0	77.0
ndhH-rps15_p5	GGTGGTGAGGTCCCATATTG	CGGAAAACGCCAACGATTAC	55.0	50.0	57.7	58.1	264 bp	1.0	76.8