Hotspot

accD-psaI

LSC · 61813-62561 · 749 bp

Back to Siraitia

Pi 0.00538

MarkerSeek score 62.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 749
variable_sites: 4
pi: 0.00538
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GCTTTCGTTCCCTCGAATCA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.3	58.0	853-858 bp	1.0	78.4
accD-psaI_p2	GCTTTCGTTCCCTCGAATCA	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.3	57.9	859-864 bp	1.0	77.8
accD-psaI_p3	GAGTTATTTCAGCTCCACGC	CCATTGCAATTGCCGGAAAT	50.0	45.0	56.9	58.0	871-876 bp	1.0	71.8
accD-psaI_p4	GCTTTCGTTCCCTCGAATCA	CCGGAAATACTAGGCCCACT	50.0	55.0	58.3	58.9	841-846 bp	1.0	71.7
accD-psaI_p5	GAGTTATTTCAGCTCCACGC	AAGAAGCCATTGCAATTGCC	50.0	45.0	56.9	57.9	877-882 bp	1.0	71.2