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psbZ-trnG-GCC

LSC · 38345-38944 · 600 bp

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Pi 0.00702

MarkerSeek score 67.60

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 600
variable_sites: 4
pi: 0.00702
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTTCTCCTGATGGTTGGTCG	AATCGAACCCGCATCTTCTC	55.0	50.0	58.0	58.1	762-765 bp	1.0	83.0
psbZ-trnG-GCC_p2	CTTCTCCTGATGGTTGGTCG	GAATCGAACCCGCATCTTCT	55.0	50.0	58.0	58.1	763-766 bp	1.0	83.0
psbZ-trnG-GCC_p3	CTTCTCCTGATGGTTGGTCG	CATTATCCTGATCCTGGGCG	55.0	55.0	58.0	57.9	791-794 bp	1.0	82.5
psbZ-trnG-GCC_p4	GATGGTTGGTCGGGTAACAA	AATCGAACCCGCATCTTCTC	50.0	50.0	57.8	58.1	754-757 bp	1.0	81.6
psbZ-trnG-GCC_p5	GATGGTTGGTCGGGTAACAA	GAATCGAACCCGCATCTTCT	50.0	50.0	57.8	58.1	755-758 bp	1.0	81.6