Hotspot

trnT-GGU-psbD

LSC · 33983-35057 · 1075 bp

Back to Siraitia

Pi 0.0103

MarkerSeek score 65.20

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1075
variable_sites: 11
pi: 0.0103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1225-1250 bp	1.0	78.6
trnT-GGU-psbD_p2	GTGGTAGAGTAACGCCATGG	GACCGGACCAACCTACAAAA	55.0	50.0	58.1	57.7	1239-1264 bp	1.0	78.5
trnT-GGU-psbD_p3	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1228-1253 bp	1.0	78.4
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	GACCGGACCAACCTACAAAA	50.0	50.0	58.1	57.7	1242-1267 bp	1.0	78.3
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	TAGACCGGACCAACCTACAA	55.0	50.0	58.1	57.7	1241-1266 bp	1.0	78.1