Hotspot

trnK-UUU-rps16

LSC · 4199-5022 · 824 bp

Back to Siraitia

Pi 0.00492

MarkerSeek score 62.90

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 824
variable_sites: 4
pi: 0.00492
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTCGCGGTCTTACAAACTC	AGAAGGCAGGGGTTTTTACG	50.0	50.0	57.9	58.1	982-1147 bp	1.0	79.9
trnK-UUU-rps16_p2	GTCGCGGTCTTACAAACTCT	AGAAGGCAGGGGTTTTTACG	50.0	50.0	57.9	58.1	981-1146 bp	1.0	79.9
trnK-UUU-rps16_p3	CAGTCGCGGTCTTACAAACT	AGAAGGCAGGGGTTTTTACG	50.0	50.0	58.2	58.1	983-1148 bp	1.0	78.6
trnK-UUU-rps16_p4	TCAGTCGCGGTCTTACAAAC	AGAAGGCAGGGGTTTTTACG	50.0	50.0	58.2	58.1	984-1149 bp	1.0	78.6
trnK-UUU-rps16_p5	CCGCACTTAAAAGCCGAGTA	AGAAGGCAGGGGTTTTTACG	50.0	50.0	58.3	58.1	908-1073 bp	1.0	78.2