Hotspot

psbA-trnK-UUU

LSC · 1332-1630 · 299 bp

Back to Siraitia

Pi 0.0337

MarkerSeek score 62.40

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 299
variable_sites: 10
pi: 0.0337
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	AAACGACCCCATAGGCTTTC	ACTCCATCCGACTAGTTCCG	50.0	55.0	57.9	58.6	389-391 bp	1.0	76.6
psbA-trnK-UUU_p2	GAAACGACCCCATAGGCTTT	ACTCCATCCGACTAGTTCCG	50.0	55.0	57.9	58.6	390-392 bp	1.0	76.6
psbA-trnK-UUU_p3	CCAGTTACAGAAACGACCCC	ACTCCATCCGACTAGTTCCG	55.0	55.0	58.2	58.6	399-401 bp	1.0	76.0
psbA-trnK-UUU_p4	AAACGACCCCATAGGCTTTC	ACTAGTTCCGGGTTCGAGTC	50.0	55.0	57.9	58.8	379-381 bp	1.0	74.9
psbA-trnK-UUU_p5	ACGGTTTTCAGTGCTGGTTA	ACTCCATCCGACTAGTTCCG	45.0	55.0	57.7	58.6	420-422 bp	1.0	74.7