Hotspot

ycf1

SSC · 121943-127453 · 5511 bp

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Pi 0.0131

MarkerSeek score 46.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5511
variable_sites: 72
pi: 0.0131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCGTCCAACCTCAATTGAA	CCCTAAAGCGTCCCATATCG	45.0	55.0	58.0	57.9	389 bp	1.0	81.2
ycf1_p2	TTGCGTCCAACCTCAATTGA	CCCTAAAGCGTCCCATATCG	45.0	55.0	58.0	57.9	390 bp	1.0	81.2
ycf1_p3	GTTGCGTCCAACCTCAATTG	CCCTAAAGCGTCCCATATCG	50.0	55.0	57.9	57.9	391 bp	1.0	81.0
ycf1_p4	TGCGTCCAACCTCAATTGAA	GCGTCCCATATCGAATCTGG	45.0	55.0	58.0	58.3	382 bp	1.0	79.6
ycf1_p5	TTGCGTCCAACCTCAATTGA	GCGTCCCATATCGAATCTGG	45.0	55.0	58.0	58.3	383 bp	1.0	79.6