Hotspot

psbA

LSC · 317-1375 · 1059 bp

Back to Siphocranion

Pi 0.00189

MarkerSeek score 49.90

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1059
variable_sites: 2
pi: 0.00189
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	ACTCCTACACACTAAAAACAAGTC	AAATTGGTGTGCTTGGGAGT	37.5	45.0	56.8	57.9	1134 bp	1.0	40.1
psbA_p2	ACTCCTACACACTAAAAACAAGT	AAATTGGTGTGCTTGGGAGT	34.8	45.0	55.7	57.9	1134 bp	1.0	40.1
psbA_p3	AGGGGTTTATTGCTCCTTAT	AAATTGGTGTGCTTGGGAGT	40.0	45.0	53.6	57.9	1177 bp	0.5	24.3
psbA_p4	AGGGGTTTATTGCTCCTTAT	AATTGGTGTGCTTGGGAGTC	40.0	50.0	53.6	58.4	1176 bp	0.5	21.9
psbA_p5	AGGGGTTTATTGCTCCTTAT	GAAAATTGGTGTGCTTGGGAG	40.0	47.6	53.6	58.0	1179 bp	0.5	20.0