Hotspot

ccsA

SSC · 112611-113588 · 978 bp

Back to Siphocranion

Pi 0.00818

MarkerSeek score 49.70

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 978
variable_sites: 8
pi: 0.00818
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TAGACACGCTGCTCTTAGGA	AACGGGGCCCTTTTACAAAT	50.0	45.0	57.9	58.0	1870 bp	1.0	80.0
ccsA_p2	ACAAGCCGCTATGGTGAAAT	AACGGGGCCCTTTTACAAAT	45.0	45.0	57.9	58.0	1893 bp	1.0	79.9
ccsA_p3	TAGACACGCTGCTCTTAGGA	TCAGTAGCTTTTCGATGGCC	50.0	50.0	57.9	58.0	1722 bp	1.0	79.9
ccsA_p4	ACAAGCCGCTATGGTGAAAT	TCAGTAGCTTTTCGATGGCC	45.0	50.0	57.9	58.0	1745 bp	1.0	79.9
ccsA_p5	TAGACACGCTGCTCTTAGGA	CAGTAGCTTTTCGATGGCCT	50.0	50.0	57.9	58.0	1721 bp	1.0	79.9