Hotspot

trnL-UAG-ccsA

SSC · 112509-112610 · 102 bp

Back to Siphocranion

Pi 0.0392

MarkerSeek score 70.80

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 102
variable_sites: 4
pi: 0.0392
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TAGACACGCTGCTCTTAGGA	AGAATTGAAACAGTCGAAAAAGA	50.0	30.4	57.9	55.0	222-223 bp	1.0	41.4
trnL-UAG-ccsA_p2	AAATTGGTAGACACGCTGCT	AGAATTGAAACAGTCGAAAAAGA	45.0	30.4	57.8	55.0	229-230 bp	1.0	41.4
trnL-UAG-ccsA_p3	GGTAGACACGCTGCTCTTAG	AGAATTGAAACAGTCGAAAAAGA	55.0	30.4	57.8	55.0	224-225 bp	1.0	41.4
trnL-UAG-ccsA_p4	GTAGACACGCTGCTCTTAGG	AGAATTGAAACAGTCGAAAAAGA	55.0	30.4	57.8	55.0	223-224 bp	1.0	41.4
trnL-UAG-ccsA_p5	ACAAGCCGCTATGGTGAAAT	AGAATTGAAACAGTCGAAAAAGA	45.0	30.4	57.9	55.0	245-246 bp	1.0	41.3