Hotspot

rpl32-trnL-UAG

SSC · 111765-112431 · 667 bp

Back to Siphocranion

Pi 0.0255

MarkerSeek score 65.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 667
variable_sites: 17
pi: 0.0255
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CTTTGGGGCGGCATTAAAAG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	806-907 bp	1.0	80.5
rpl32-trnL-UAG_p2	CTTTGGGGCGGCATTAAAAG	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.0	57.8	799-900 bp	1.0	80.1
rpl32-trnL-UAG_p3	CTTTGGGGCGGCATTAAAAG	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.0	57.8	804-905 bp	1.0	80.0
rpl32-trnL-UAG_p4	CTTTGGGGCGGCATTAAAAG	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	58.0	57.8	805-906 bp	1.0	79.9
rpl32-trnL-UAG_p5	CTTTGGGGCGGCATTAAAAG	GAGCAGCGTGTCTACCAATT	50.0	50.0	58.0	58.3	800-901 bp	1.0	79.3