Hotspot

petA-psbJ

LSC · 62827-63750 · 924 bp

Back to Siphocranion

Pi 0.0162

MarkerSeek score 54.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 924
variable_sites: 15
pi: 0.0162
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGGCCGAAATGAATTTCTAG	TCCGGATTAGGTTCATCCCT	40.0	50.0	53.7	57.5	968 bp	1.0	42.7
petA-psbJ_p2	TGGCCGAAATGAATTTCTAG	CCGGATTAGGTTCATCCCTG	40.0	55.0	53.7	57.4	967 bp	1.0	42.2
petA-psbJ_p3	TGGCCGAAATGAATTTCTAG	TTCCGGATTAGGTTCATCCCT	40.0	47.6	53.7	58.2	969 bp	1.0	41.2
petA-psbJ_p4	TGGCCGAAATGAATTTCTAG	CGGATTAGGTTCATCCCTGT	40.0	50.0	53.7	56.7	966 bp	1.0	41.2
petA-psbJ_p5	TGGCCGAAATGAATTTCTAG	TTCCGGATTAGGTTCATCCC	40.0	50.0	53.7	56.7	969 bp	1.0	41.2