Hotspot

trnS-UGA-psbZ

LSC · 35051-35403 · 353 bp

Back to Siphocranion

Pi 0.0129

MarkerSeek score 63.30

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 353
variable_sites: 4
pi: 0.0129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAACCACTCAGCCATCTCTC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.0	57.8	430-473 bp	1.0	82.9
trnS-UGA-psbZ_p2	CAACCACTCAGCCATCTCTC	GACCAACCATCAGGAGAAGC	55.0	55.0	58.0	58.3	432-475 bp	1.0	82.5
trnS-UGA-psbZ_p3	GTTTTCAAGACCGGGGCTAT	CCAACCATCAGGAGAAGCAA	50.0	50.0	57.9	57.8	450-493 bp	1.0	82.0
trnS-UGA-psbZ_p4	CAACCACTCAGCCATCTCTC	GCAAATACGACAGGTACGCT	55.0	50.0	58.0	58.4	414-457 bp	1.0	81.7
trnS-UGA-psbZ_p5	GTTTTCAAGACCGGGGCTAT	GACCAACCATCAGGAGAAGC	50.0	55.0	57.9	58.3	452-495 bp	1.0	81.6