Hotspot

trnT-GGU-psbD

LSC · 30953-32274 · 1322 bp

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Pi 0.0146
MarkerSeek score 68.90
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1322
variable_sites
19
pi
0.0146
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG CAGCCTACAAAAACGAAGCG 55.0 50.0 58.1 58.0 1468-1479 bp 1.0 81.5
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA CAGCCTACAAAAACGAAGCG 50.0 50.0 58.1 58.0 1471-1482 bp 1.0 81.4
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG GGACCAGCCTACAAAAACGA 55.0 50.0 58.1 58.1 1472-1483 bp 1.0 81.0
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA GGACCAGCCTACAAAAACGA 50.0 50.0 58.1 58.1 1475-1486 bp 1.0 80.8
trnT-GGU-psbD_p5 ACATAGTGACTCGTTCGGGA CAGCCTACAAAAACGAAGCG 50.0 50.0 58.2 58.0 1516-1527 bp 1.0 80.6