Hotspot

rps16-trnQ-UUG

LSC · 5851-6693 · 843 bp

Back to Siphocranion

Pi 0.0261

MarkerSeek score 62.10

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 843
variable_sites: 22
pi: 0.0261
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.2	58.1	908-911 bp	1.0	75.0
rps16-trnQ-UUG_p2	GCACGTTGCTTTCTACCACA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	59.1	58.1	911-914 bp	1.0	72.9
rps16-trnQ-UUG_p3	CGTTGCTTTCTACCACATCG	GGGTTTTGGTCCCGGTATTC	50.0	55.0	57.2	58.5	929-932 bp	1.0	71.3
rps16-trnQ-UUG_p4	CGTTGCTTTCTACCACATCG	TTCGGAGGTTCGAATCCTTC	50.0	50.0	57.2	57.3	912-915 bp	1.0	70.3
rps16-trnQ-UUG_p5	CGTTGCTTTCTACCACATCG	TAAGGCAACGGGTTTTGGTC	50.0	50.0	57.2	58.7	938-941 bp	1.0	70.1