Hotspot

ycf1

SSC · 121883-126970 · 5088 bp

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Pi 0.0125

MarkerSeek score 50.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5088
variable_sites: 148
pi: 0.0125
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTGTAGAGCTATTCTGCGCA	ATCCTTTTGGTCCTCCTCGA	45.0	50.0	57.0	58.0	533-542 bp	0.857143	70.5
ycf1_p2	TTGTAGAGCTATTCTGCGCA	CAGATTTTCGTCGAGCCGTA	45.0	50.0	57.0	58.1	1169-1184 bp	0.857143	69.3
ycf1_p3	TTGTAGAGCTATTCTGCGCA	TCGTCGAGCCGTAATCAAAG	45.0	50.0	57.0	58.1	1162-1177 bp	0.857143	69.3
ycf1_p4	TTGTAGAGCTATTCTGCGCA	TGGTCCTCCTCGAAAAACAC	45.0	50.0	57.0	57.8	526-535 bp	0.857143	69.0
ycf1_p5	AGTTGTAGAGCTATTCTGCGC	ATCCTTTTGGTCCTCCTCGA	47.6	50.0	58.2	58.0	535-544 bp	0.857143	68.7