Hotspot

atpA

LSC · 28109-29635 · 1527 bp

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Pi 0.00259

MarkerSeek score 35.30

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 10
pi: 0.00259
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	TTACATTGAGCGTGAGAGCC	AGCCTTCCAAGCTAACGATG	50.0	50.0	58.0	58.0	2513-2595 bp	1.0	82.8
atpA_p2	TTACATTGAGCGTGAGAGCC	CCTAGCCTTCCAAGCTAACG	50.0	55.0	58.0	58.1	2516-2598 bp	1.0	82.6
atpA_p3	TCATGTTTGGTTCGGGAAGG	AGCCTTCCAAGCTAACGATG	50.0	50.0	58.1	58.0	2477-2559 bp	1.0	82.2
atpA_p4	AGCGTGAGAGCCAAATGAAT	AGCCTTCCAAGCTAACGATG	45.0	50.0	57.9	58.0	2505-2587 bp	1.0	82.0
atpA_p5	ATTGAGCGTGAGAGCCAAAT	AGCCTTCCAAGCTAACGATG	45.0	50.0	57.9	58.0	2509-2591 bp	1.0	82.0