Hotspot

ndhC-trnV-UAC

LSC · 50289-51062 · 774 bp

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Pi 0.0143
MarkerSeek score 67.00
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
774
variable_sites
25
pi
0.0143
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCGGAGCTAAAAATCCCGAA AGAAGGTCTACGGTTCGAGT 45.0 50.0 56.9 57.8 869-907 bp 1.0 76.2
ndhC-trnV-UAC_p2 TCGGAGCTAAAAATCCCGAAA AGAAGGTCTACGGTTCGAGT 42.9 50.0 57.7 57.8 869-907 bp 1.0 74.0
ndhC-trnV-UAC_p3 TCGGAGCTAAAAATCCCGAA GAAGGTCTACGGTTCGAGTC 45.0 55.0 56.9 57.5 868-906 bp 1.0 73.4
ndhC-trnV-UAC_p4 TCGGAGCTAAAAATCCCGAA GTTCGAGTCCGTATAGCCCT 45.0 55.0 56.9 58.7 857-895 bp 1.0 72.3
ndhC-trnV-UAC_p5 TCGGAGCTAAAAATCCCGAAA GAAGGTCTACGGTTCGAGTC 42.9 55.0 57.7 57.5 868-906 bp 1.0 71.2